LOCUS       40924_12760        4679 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway conversion vector pCON-Cm-rare1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Kimura T, Nakao A, Murata S, Kobayashi Y, Tanaka Y, Shibahara K, 
            Kawazu T, Nakagawa T.
  TITLE     Development of the gateway recycling cloning system for multiple 
            linking of expression cassettes in a defined order, and direction on
            gateway compatible binary vectors
  JOURNAL   Biosci. Biotechnol. Biochem. 77 (2), 430-434 (2013)
  PUBMED    23391940
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Kimura T, Shibahara K, Tanaka Y, Nakao A, Kawazu T, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 4679)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4679)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2013"; volume: "77"; issue: "2"; 
            pages: "430-434"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-2012) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pDONR201.
FEATURES             Location/Qualifiers
     source          1..4679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    28..127
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     652..668
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    676..800
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    801..808
                     /label=rare cutter site
                     /note="rare cutter site"
     promoter        825..855
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             909..1565
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    complement(1927..2051)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2552..2651)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2774..3580
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3726..4314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(4449..4476)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4568..4654)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc
       61 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtatagaaaa
      121 gtaggctgga tgaatcgaat aacttcttct tcaaatgcgc aaacgaagaa atttcctcag
      181 tctcagccac agccgccgga gaaaaatgag aaaaaactga aaagggagag atttccggtg
      241 aaccctttga gagatttcag tacaagaagc tctggaagca gcagttgtag caatgagagt
      301 gcttctggtt caacttccgg tgaagcatcc aacggttgtc acaggttctt gctctctcat
      361 tctttctcat cgtcttcttc atcatcttct tcttcgttgg gagtttttcc tcgtagacca
      421 gtgagatcag tagctaaaaa ccccaaatct gctccggtag ttagtaaacc cttaattaga
      481 aagaaaccaa gctctcttga ggaggttaag ttgaaatcta ctcttactga gaaacctaat
      541 ttgctgaagt ctcagagatg caagacaaat cctgtatctg gaaagagacc aacttgtaag
      601 attaccatga aaccggagaa agtttcggtt ttgaagaaac aatcttctgt ttctagaact
      661 agtggatccc ccatcacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
      721 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
      781 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
      841 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
      901 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
      961 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
     1021 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
     1081 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
     1141 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
     1201 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
     1261 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
     1321 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
     1381 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
     1441 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg
     1501 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
     1561 gggcgtaatc gcgtggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
     1621 ctgatttttg cggtataaga atatatactg atatgtaaac gggcgacgga tggtgatccc
     1681 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     1741 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     1801 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     1861 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     1921 atcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     1981 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     2041 tacaaagtgg tgatatcaag cttcgacaat tattttggac tctcatgttc tcaagctgaa
     2101 atccaaaaga aacatgatgt tggttcagat ttggaatcac tgacaatgaa gtttcagtca
     2161 gccggtttgt caccaaggat ccaggcttca tcatgggagc cgagccgttc aagtttcaac
     2221 ttcgactatt tggccacatc ttctgattct attgatctct cacagtttca gagaggttta
     2281 gttgacagga gttcttgtca tcctcatgta acactggata aagtgtcaag gacacatttg
     2341 agagtagagc aaacaaatag ccacatgcct gaaatgaaat cccaacagat tacagatact
     2401 gaatttgata ttcaaaatca caaagaaagt gctgcttctc tgggaaaagg aagagaattg
     2461 cttccatgct ctgctgcaga gtcaataagc actgatggag gtggtctgat ttgttctgag
     2521 gattcaaatt ggatggcatg ttacaagaat tacccaactt tattatacaa agttggcatt
     2581 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2641 atcattattt gccatccagc tgcagctctg gcccgtgtct caaaatctct gatgttacat
     2701 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2761 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2821 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2881 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2941 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3001 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3061 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3121 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3181 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3241 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3301 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3361 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3421 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3481 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3541 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3601 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3661 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3721 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3781 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3841 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     3901 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3961 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     4021 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     4081 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     4141 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     4201 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     4261 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     4321 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4381 cctgcgttat cccctgattc tgtggataac cgtattaccg ctagccagga agagtttgta
     4441 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     4501 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     4561 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     4621 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgc
//
